Mechanism of Regulating the Proliferation, Migration and Invasion of Cervical Cancer Hela Cells by Circular RNA Circ_0025033 Targeting microRNA 1256

To explore the effect of circular RNA_0025033 on proliferation, migration and invasion cervical cancer HeLa cells its molecular mechanism. The tissues adjacent 39 patients with in our hospital from January 2017 to December 2020 were selected. expression levels microRNA-1256 detected by Quantitative reverse transcription polymerase chain reaction. Cell counting kit 8 was used detect cell viability. Plate cloning test conducted number clones. Scratch carried out scratch healing rate. Transwell chamber invasion. Western blot adopted protein expression. Dual luciferase reporter assay targeting relationship between microRNA-1256. level increased decreased (p<0.05). After inhibiting or overexpression microRNA-1256, viability clone formation decreased. rate scratches invasive decrease. E-cadherin N-cadherin Circular targeted regulation out; interference reversed inhibition cells. Inhibition may inhibit through